SEM-CLSM CORRELATION MICROSCOPY AND ITS APPLICATION TO ELECTROSPUN GELATIN FIBERS
The most comprehensive information about microstructure of the sample can be obtained by combining different types of high-resolution microscopy. This combination turns out to be especially informative when measurements are carried out not only on the same image, but on the same area of the sample. This approach is called correlation microscopy. Typically, such experiments require careful preparation of the sample and transferring it between the two microscopes. The current work uses correlation microscopy which combines scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Electrospun gelatin fibers deposited onto metallized glass are studied using these two methods. The possibility of using correlation analysis to combine images obtained by SEM and CLSM is demonstrated.
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